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Journal of Dairy Science Vol. 46 No. 1 57-60
© 1963 by American Dairy Science Association ®
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Livability of Bovine Spermatozoa at 5 C in Tris-Buffered and Citrate-Buffered Yolk-Glycerol Extenders

I. S. Davis1, R. W. Bratton and R. H. Foote

Department of Animal Husbandry, Cornell University, Ithaca, New York

ABSTRACT

When bovine spermatozoa were stored in 20% egg yolk-glycerol extenders buffered with .15, .2, and .25 M Tris (hydroxymethyl) aminomethane and adjusted with citric acid to pH's of 6.50, 6.75, and 7.00 optimum sperm survival was found in the 0.2 M extender with a pH of 6.75. After eight days of storage at 5 C the motile sperm in this extender averaged 42%, compared with 35% in CUE and 18% in a citrate-yolk-glycerol extender used routinely for freezing bovine spermatozoa.

FOOTNOTES

1 Present address: Cornell University Medical College, New York, New York.






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Copyright © 1963 by the American Dairy Science Association ®.