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Determination of -Lactalbumin in Complex Systems¹

Laboratory of Biochemistry, Department of Dairy Science, University of Illinois, Urbana

ABSTRACT

A simple method based on an immunological procedure has been devised to quantitate accurately the amount of -lactalbumin present in complex systems including milk and milk products. The method is based on the rate of diffusion in a small tube of -lactalbumin into a layer of agar containing antisera to the -lactalbumin. The techniques were originally described by Oudin and recently adapted in this laboratory for the quantitative determination of ß-lactoglobulin in similar complex systems. The usable range of the method is about 6 to 80 µg/ml of -lactalbumin. Milk contains about 1,000 to 1,500 µg/ml of -lactalbumin; thus, dilution is the only sample preparation required for milk and milk products. A concentration procedure has also been devised with which it has been possible to determine less than 1 µg/ml of -lactalbumin in complex solutions containing high levels of blood and other cellular proteins.

¹ This investigation was supported in part by a grant from the National Science Foundation.