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Survival of Bull Sperm in Milk and Yolk Extenders with Added Catalase

Department of Animal Husbandry, Cornell University, Ithaca, New York

ABSTRACT

After 12 days of storage at 5 C the percentage of motile sperm in CUE containing 0, 1, 10, 100, and 200 µg of catalase per milliliter of extender was 28, 37, 44, 44, and 45, in CU-16 was 35, 43, 47, 52, and 50, in CU-16 plus chlorpromazine hydrochloride was 29, 35, 35, 41, and 43, and in 20% yolk-citrate was 24, 34, 38, 37, and 34, respectively. All levels of catalase improved sperm survival significantly (P < .01), but little additional response was observed with catalase levels higher than 10 µg/ml of extender. The concentration of catalase required to produce a maximum response was much greater than that theoretically required to eliminate any hydrogen peroxide produced. No beneficial effect of adding 20 µg of catalase per ml of skimmilk or skimmilk-glycerol extender was found. The percentage of motile sperm after 12 days of storage at 5 C averaged 47 for CU-16 extender with catalase, 11 for skimmilk, and 12 for skimmilk with catalase. The reason for the differential response to catalase in egg yolk versus milk has not been elucidated.

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