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Acid-Soluble Nucleotides in Cow's and Goat's Milk¹

Laboratory of Physiology of Lactation, Division of Animal Physiology National Institute of Agricultural Sciences, Chiba, Japan

ABSTRACT

Acid-soluble nucleotides in cow's and goat's milk at different stages of lactation were examined by ion-exchange column chromatography. In goat milk CMP5', DPN, AMP5', AMP-X, GMP5', UMP5', orotic acid, GDP-mannose, UDP-acetylglucosamine, UDP-acetylgalactosamine, UDP-glucose, UDP-galactose, GDP, UDP, UDP-uronic acid, and other unidentified nucleotides were found throughout the lactation period. Total content of acid-soluble nucleotides attained maximum at the colostral stage (330 µM per 100 ml) and decreased with the advance of lactation. The percentage of UDP-acetylhexosamine and UDP-hexose in acid-soluble nucleotides are not only highest in colostrum (68%) but also in the mid-stage of lactation (48%). Active P³² incorporation into these nucleotides was observed following the injection of P³². Milk of a goat with lactation induced by the administration of hormones showed the same chromatographic pattern as that of the milk at normal lactation.

Considerable species difference of the acid-soluble nucleotides of milk was observed between cow's and goat's milk. With the exception of the colostral stage, orotic acid constituted a major part of acid-soluble nucleotides of cow milk. Cow's milk also contained CMP5', uric acid, AMP5', and an unidentified AMP derivative. There was no difference in the patterns of chromatograms between Holstein and Jersey milk.

¹ In this paper, the following abbreviations are used: P, phosphate; AMP, adenosine monophosphate; CMP, cytidine monophosphate; GMP, guanosine monophosphate; UMP, uridine monophosphate; ADP, adenosine diphosphate; GDP, guanosine diphosphate; UDP, uridine diphosphate; ATP, adenosine triphosphate; GTP, guanosine triphosphate; UTP, uridine triphosphate; DPN, diphosphopyridine nucleotide; TPN, triphosphopyridine nucleotide; G-1-P, glucose-1-phosphate; G-6-P, glucose-6-phosphate; DNA, desoxyribonueleic acid; RNA, ribonucleic acid.