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Department of Food Science
Department of Biochemistry, Michigan State University, East Lansing
ABSTRACT
A method was developed for evaluating the detergency of trisodium phosphate on milk protein films alone and in the presence of sodium hypochlorite.
Synthetic soil films were prepared by dipping mechanically 22 by 50 by 0.14–0.25 mm micro cover glasses into an aqueous milk plant clarifier slime suspension. Hardness and tenacity were imparted to the films by heating and adding trisodium phosphate, calcium chloride, and sodium hypochlorite in alternate dipping solutions. The films were dried and weighed, then exposed to a detergent treatment. Immediately after the detergent treatment the glass film supports, containing the film residues, were transferred quantitatively into 50-ml micro-Kjeldahl flasks for nitrogen determination.
When maintained at pH 11.5 ± 0.1 at a temperature of 65 to 54 C (beginning at 65 C, at the end of period the temperature was 54 C) for 10 min without agitation, the use of sodium hypochlorite with trisodium phosphate significantly increased the protein removal, using either tap or distilled water. No optimum level of hypochlorite was observed. Increased levels resulted in increased peptization.
1 Journal Article No. 2941 from the Michigan Agricultural Experiment Station.
2 Present address: Animal and Dairy Husbandry Department, The University of Vermont, Burlington, Vermont.
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