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Effect of Addition of Phosphates to Milk on Development of Bacteriophage and Growth of Lactic Cultures¹

Dairy Department, Purdue University, Lafayette, Indiana

ABSTRACT

The suppression or prevention of bacteriophage development in media by binding the calcium ions, or by the removal of calcium, is well recognized. One of the first investigations in this area involved the use of citrate as the calcium-binding agent (5). A calcium-free medium prepared from skimmilk was developed by Reiter (4) for the purpose of preventing bacteriophage development. Collins et al. (1) suggested the use of a medium prepared from chemically defined nutrients containing no calcium, or a medium low in calcium and supplemented with sufficient phosphate or other calcium-binding ions. Hargrove (2) outlined a method for limiting bacteriophage development in milk by adding phosphates to bind the calcium. Experiments conducted with this method were reported by Hargrove et al. (3).

The purpose of this investigation was to determine the effectiveness of some phosphate mixtures for binding the calcium ions in milk so that bacteriophages would not develop, and to determine the influence of the phosphates on the growth of lactic cultures.

¹ Published with the approval of the Director of the Purdue Agricultural Experiment Station as Journal Series Paper No. 1764.

² Present address: Alberta Department of Agriculture, Edmonton, Alberta, Canada.