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Survival of Bovine Spermatozoa at Room Temperature in Citrate and Cornell University and Tris Extenders Containing Whole and Fractionated Coconut Milk¹

Department of Animal Husbandry

E. M. Shantz and J. K. Pollard

Department of Botany, Cornell University, Ithaca, New York

ABSTRACT

The average percentage of motile sperm over an eight-day storage period in a citrate buffer alone or with the addition of 15% by volume of whole coconut milk (CM), neutral fraction (NF), and active fraction (AF), for carrot ex-plants, or NF + AF was 19, 34, 42, 22, and 36, respectively. Corresponding values in Cornell University Extender (CUE) were, respectively, 44, 42, 44, 42, and 42% and for Tris (hydroxymethyl) aminomethane (Tris), 31, 32, 24, 32, and 30%. Whole coconut milk and the neutral fraction equally improved sperm survival in citrate buffer, but did not improve sperm survival in the other buffers. Removal of anions and cations from the NF reduced this response, but carbohydrates or other material, or both, remaining in the NF produced some effect. The fraction active in producing cell division in carrot explants was ineffective for sperm. In general, the CUE buffer was equal or superior to the other treatments tested, indicating that coconut milk had no special properties due to unknown substances. Sperm survival in all buffers was improved by the addition of egg yolk.

¹ This joint investigation between the two departments represents, so far as it relates to coconut milk, a part of a series of investigations being supported by Grant C-1357 from the National Cancer Institute to the Department of Botany.