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Journal of Dairy Science Vol. 45 No. 10 1242-1247
© 1962 by American Dairy Science Association ®
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Motility and Fertility during Post-Thawing Storage of Bovine Spermatozoa Frozen Concentrated, Thawed, and Re-Extended1

C. Desjardins and H. D. Haes

Department of Dairy, Michigan State University, East Lansing

ABSTRACT

Twenty ejaculates of semen from 17 dairy bulls were extended to contain 200 x 106 and 20 x 106 sperm per milliliter in yolk citrate and whole milk extenders and frozen with 7% glycerol. The semen frozen with 200 x 106 sperm per milliliter was re-extended, after thawing, to 20 x 106 sperm per milliliter in yolk citrate, whole milk, skimmilk, and CUE, and the motility of these semen samples wTas estimated during 72 hr of storage at 5 C.

Freezing semen with 200 x 106 sperm per milliliter resulted in significantly improved motilities (P<.01), particularly as the storage interval increased (P<.01). The four re-extension extenders differed in their ability to maintain motility (P<.01), particularly as the storage interval increased (P<.01). In general, sperm survived post-thawing storage much better when re-extended in extenders other than those in which the sperm were frozen.

The average 60- to 90-day nonreturn percentages for semen frozen with 200 x 106 sperm per milliliter and re-extended after thawing to 15 x 106 motile sperm per milliliter in yolk-citrate and CUE was 51.8 and 63.0%, respectively, as measured with a total of 2,062 inseminations made between 24 and 60 hr after thawing. The 63.0% for CUE did not significantly differ from the average of 66.5% for control frozen semen thawed immediately before 675 cows were inseminated (P > .05), but both of these values were significantly higher than the 51.8% for the semen re-extended in yolk citrate (P < .05).

FOOTNOTES

1 Journal Article 2993 from the Michigan Agricultural Experiment Station.






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