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Sensitivity of Milk Lipase to Antibiotics¹

Department of Dairy Husbandry, University of Nebraska, Lincoln

ABSTRACT

A study was made to determine the effect of various concentrations (0.5–50.0 ppm) of antibiotics upon milk lipase. Aureomycin, penicillin, streptomycin, and terramycin inhibited the lipase activity by 9.5 to 42.4, 7.9 to 49.8, 9.2 to 39.6, and 7.6 to 44.2%, respectively. Up to 10.0 ppm, there was a direct relationship between the concentrations of the antibiotics and the per cent lipase inhibition, but with higher concentration the increase in lipase inhibition became disproportional to the antibiotic concentration. Fractional determinations of the fatty acids released showed that antibiotics did not possess any selectivity in inhibiting the release of short-, medium- or long-chain fatty acids by the enzyme. An increase in the fat content of milk lipase systems containing antibiotics nullified the inhibitory effect of antibiotics. Evidence is presented which indicates that the antibiotics apparently work as competitive inhibitors of milk lipase.

¹ Published with the approval of the Director as paper No. 1267 Journal Series, Nebraska Agricultural Experiment Station, Lincoln.