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Growth Stimulation of Butyrivibrio by Mucin

Virginia Agricultural Experiment Station, Blacksburg, Virginia

ABSTRACT

In most work involving the culture of rumen bacteria, rumen fluid has been incorporated in the media to stimulate growth, and has been found to be essential for growth of some of these organisms (1–5, 9). Several components of ruminal ingesta have been found to provide stimulation or fulfill requirements for certain rumen organisms or to stimulate cellulose digestion by cultures of rumen organisms. The active compounds include valine, proline, leucine, and isoleucine (7), branched and straight-chain fatty acids (5, 6), and several less well identified compounds. Gill and King (8) demonstrated that rumen fluid stimulated the growth of Butyrivibrio cultures in defined media.

In the present study, growth of a culture of Butyrivibrio fibrisolvens (different from the strain used by Gill and King) was stimulated by sterile rumen fluid which was added to a basal medium containing mineral salts, an amino acid mixture, glucose, and a vitamin mixture, similar to the medium of Gill and King (8). The amount of growth stimulation was determined after 22 hr. of incubation in matched tubes by measurement of turbidity in a Klett-Summerson colorimeter with a No. 66 red filter.

¹ Present address: Bacteriology Department, Research Center, Christ Hospital, Cincinnati, Ohio.