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Acid-Soluble Phosphorus Constituents of Bull Semen in Relation to Fructolysis and to Sperm Concentration^{1, 2,}

Department of Dairy Science, Washington State University, Pullman

ABSTRACT

Eighty-seven semen samples from five bulls had an average phosphorus content
of 0.807 mg. per milliliter and these acid-soluble phosphorus values in milligrams per milliliter: 0.039 inorganic (PI), 0.046 inorganic + easily hydrolyzable (PI+H), and 0.460 total acid-soluble (PTAS). During 3 hr. of incubation of these samples in citrate buffer, PI increased 0.013 (P < 0.01); PI+H increased 0.008 (P < 0.01); but PTAS did not significantly change. Increase in PI was positively correlated with sperm concentration, but negatively correlated with fructose utilization. Thirty-two of these samples, based on hourly incubation data, so depleted their fructose content during the second or third hour (< 0.25 mg. per milliliter incubate remaining) that utilization was halved, compared with the hour prior to this depletion. PI, however, showed a similar increase during both hours of comparison. Fifteen samples adjusted with seminal plasma to %, 1, and 2 x the original sperm concentration indicated that during 3 hr. of incubation increase in PI was largely, but not wholly, a function of sperm numbers, where sperm were from a similar source. The data do not give conclusive evidence as to the source of increase in PI in bull semen, but they do not contradict the concept that the increase is partially due to irreversible breakdown of ATP. Coupled with a report of PI increase upon cold shock of ram sperm, it is suggested that the increase indicates degenerative or senescent change.

¹ Scientific Paper No. 2042, Washington Agricultural Experiment Stations, Pullman. Station Project 1344.

² This research was financed in part under Regional Research and Marketing Act, Section963, Public Law 733. Project W-49.