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Reproductive Capacity of Dairy Bulls. I. Technique for Direct Measurement of Gonadal and Extra-Gonadal Sperm Reserves¹

Dairy Breeding Research Center, Department of Dairy Science The Pennsylvania State University, University Park

ABSTRACT

A technique for determining the gonadal and extra-gonadal sperm reserves of bulls by direct counting is described. Sperm were liberated from the epididymis by thoroughly tearing apart the tissue in 50 ml. of 0.9% NaCl and grinding the tissue suspension in 200 to 500 ml. of saline for a total of 2.5 min. in a Waring Blendor. After removing the tunica albuginea, testicular sperm were liberated by cutting the testis into small pieces and grinding in 1,000 ml. of saline for 2 min. Sperm were liberated from the ductus deferens and ampulla by flushing with distilled water to remove most of the sperm and then grinding in a blendor. Soaking the sperm suspensions for 48 hr. before counting did not result in higher values; differences between 0- and 48-hr. counts were relatively small. Sampling of the sperm suspension is the major source of error; therefore, replicate counts should be made on separate subsamples of the entire suspension.

¹ Authorized for publication on March 21, 1961, as Paper No. 2543 in the journal series of The Pennsylvania Agricultural Experiment Station.

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