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Journal of Dairy Science Vol. 44 No. 6 1143-1145
© 1961 by American Dairy Science Association ®
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Inhibition of the Spermicidal Factor(s) in Unheated Skimmilk by the Addition of Egg Yolk1

B. W. Pickett and W. A. Cowan

Department of Animal Industries University of Connecticut, Storrs

ABSTRACT

Fresh milk, skim or whole, raw or pasteurized, must be heated or chemically treated with compounds containing reactive sulfhydryl groups (SH) to support extended livability of bovine spermatozoa (1,3,8,9,12). Complete protection is apparently afforded by the addition of SH compounds and, in some cases, the motility of spermatozoa extended in chemically treated milk exhibited greater motility than split-samples extended in heated milk (3,8,9). The spermicidal factor(s) in milk and the possible role of SH in supporting motility have been investigated (6,8,10,12). In regard to fertility, Hayden et al. (7) and Bartoff (2) have reported favorable results with extenders containing added glutathione.

While several studies have dealt with mixtures of heated milk plus egg yolk, with or without glycerol, as a diluent for bull semen, some of these indicated that extenders of this type are excellent for suporting spermatozoan motility, but inferior in maintaining fertility (4,11,14).

In view of the observations that SH-treated milk and heated milk plus egg yolk provided an excellent environment for maintenance of motility, an experiment was conducted to study the effect on spermatozoal motility of adding various levels of cysteine hydrochloride (cysteine HCl) to an unheated skimmilk-egg yolk diluent.

FOOTNOTES

1 Supported in part by grants-in-aid from the Chas. H. Hood Dairy Foundation and the New England Artificial Breeding Council.






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Copyright © 1961 by the American Dairy Science Association ®.