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Correlation between Certain Laboratory Stress Tests and Fertility of Frozen Bovine Spermatozoa¹

Department of Animal Industries, University of Connecticut, Storrs

ABSTRACT

In an initial study, involving 18 ejaculates from ten Holstein bulls, spermatozoa stored in liquid nitrogen (LN) were more resistant to the imposed repeated exposure to ambient temperature, stress tests, than spermatozoa stored at dry ice-alcohol (DI) temperatures. Significant positive correlations (P < 0.05) of between 0.46 to 0.58 were obtained between stress tests and fertility. Results from the second study, with ten ejaculates obtained from seven Holstein bulls, showed that semen stored in DI for 2 hr., thawed and examined at 0, 1, 3, 6, and 12 hr. post-thaw, exhibited greater motility than split samples stored in LN. However, after 90 and 180 days of storage, the LN stored semen was superior to DI storage. The most consistent significant correlations (P < 0.05) were found at 1, 3, and 6 hr. post-thaw from DI and at 0, 1, and 3 hr. from LN after 2 hr. of storage.

¹ Supported in part by grants-in-aid from the Chas. H. Hood Dairy Foundation, Connecticut Artificial Breeding Association, and the New England Artificial Breeding Council. Portions of these data were presented at the 34th Annual Meeting of the Eastern Division of A.D.S.A., August 8–9, 1960, University of New Hampshire, Durham.

² Storrs Agricultural Experiment Station Biometrician.