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Journal of Dairy Science Vol. 44 No. 3 393-400
© 1961 by American Dairy Science Association ®
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Quantitative Determination of Lactase

Lenore Jasewicz and Aaron E. Wasserman

Eastern Regional Research Laboratory, USDA, Philadelphia 18, Pennsylvania

ABSTRACT

A simple and quick assay is described for the determination of the lactase activity of crude enzyme preparations. Results are available in 2 hr. from as little as 2 mg. of material. Fifteen minutes' incubation at 37° C. is used for the enzymatic reaction on a pure lactose substrate. The lactase activity is related to the amount of glucose released by the hydrolysis, as determined by an enzymatic micro-method. The effects of substrate concentration, pH, temperature, and related factors are investigated. The assay procedure was advantageously employed in a preliminary quantitative screening of lactaseproducing organisms which could utilize whey in their growth.






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Copyright © 1961 by the American Dairy Science Association ®.