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Amino Acid and Vitamin Composition of Saccharomyces Fragilis Grown in Whey

Eastern Regional Research Laboratory,¹ Philadelphia 18, Pennsylvania

ABSTRACT

Saccharomyces fragilis grown in a whey medium was analyzed for amino acid and vitamin contents. Qualitatively, the amino acid composition of S. fragilis was similar to that reported for other yeasts. The quantity of the individual amino acids, however, was greater in the whey-grown S. fragilis. Lysine, in particular, was present at the level of 11.14 g/16 g. N, compared to the range of 3.8 to 6.9 g/16 g. N found in other microorganisms.

An extraction procedure applied to whole, dried S. fragilis cells removed 40% of the total weight and 28% of the N of the cell preparation. The amino acid composition of the residual protein fraction was the same as that of the whole cells, hut the amounts of the component amino acids varied from 35% of the histidine to 100% of the serine and valine in the whole cell preparation.

Thiamine, pyridoxine, riboflavin, niacin, folic acid, pantothenic acid, p-aminobenzoic acid, choline, inositol, and biotin were present in S. fragilis in concentrations within the ranges reported for other microorganisms.

¹ Eastern Utilization Research and Development Division, Agricultural Research Service, U. S. Department of Agriculture.