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Lipid Phosphorus and Nucleic Acid Phosphorus Content of Bull Semen during Aerated and Nonaerated Incubation^{1, 2,}

Department of Dairy Science, Washington State University, Pullman

ABSTRACT

Seventeen samples of semen from six bulls had an average lipid phosphorus (PLIPID) content of 0.068 mg. per milliliter and an average nucleic acid phosphorus (PNUCLEIC ACID) content of 0.132 mg. per milliliter. Incubation of these samples as a thin layer in Erlenmeyer flasks (aerated) or in culture tubes (nonaerated) failed to decrease significantly PLIPID or PNUCLEIC ACID or to increase total acid-soluble phosphorus (PTAS). Inorganic phosphorus (PI) increased with both aerated and nonaerated incubation, as previously reported with nonaerated incubation. Although the source of the increase was not established and apparently did not orginate from PLIPID or PNUCLEIC ACID, there is no conflict, at least in nonaerated incubation, with the hypothesis of ATP breakdown.

¹ Scientific Paper No. 2137 Washington Agricultural Experiment Stations, Pullman, Stations Project 1344.

² The research was financed in part under Regional Research and Marketing Act Section 963 Public Law 733. Project W-49.