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Department of Dairy Science Washington State University, Pullman
ABSTRACT
Although Leuconostoc citrovorum and Leuconostoc dextranicum are important species of dairy starters, because of their role in the production of biacetyl, little information is available on the nutritional requirements of these species. Limited studies (1–4), involving only a few strains, have been made of the amino acid requirements of these species. One of the strains studied, L. citrovorum ATCC 8081, later was shown to be a typical strain of Pediococcus cerevisiae rather than a strain of L. citrovorum (2).
This study was undertaken with the hope that additional information concerning the amino acid requirement would be useful in developing a plating medium by which L. citrovorum and L. dextranicum could be differentiated in cultured dairy products.
The data presented in Table 1 represent a study of 18 strains of L. citrovorum and 15 strains of L. dextranicum isolated from dairy starters and cultured milk products.
The basal synthetic medium was that used by Whiteside-Carlson and Rosano (3), with the addition of cystine and glutamine.
1 This investigation was supported in part by funds provided for Biological and Medical Research by the State of Washington, Initiative Measure No. 171.
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