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Fatty Acid Composition of the Diglycerides from Lipolyzed Milk Fat¹

Department of Animal Industries, Storrs Agricultural Experiment Station University of Connecticut, Storrs

ABSTRACT

The diglycerides in eight samples of lipolyzed milk were isolated by silicic acid column and thin-layer chromatography and identified by infrared spectrophotometry. The average recovery of diglycerides was 93.3%. Controls contained negligible quantities. Fatty acids, identified by gas-liquid chromatography, were: palmitate, 29.5%; oleate, 24.4%; myristate, 13.2%; stearate, 8.4%; and at least 16 others commonly found in milk fat. The fatty acid composition of the diglycerides was different in some respects from that in the intact control milk fat. About 70% of the monoglycerides was recovered and similar data are reported for these compounds.

¹ Presented at the 1961 A.D.S.A. meeting, Madison, Wisconsin.

² Present address: Department of Pathology, Albany Medical School, Albany 8, N. Y.