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Establishing the Presence of Distearyl Triglycerides in a High-Melting Fraction of Milk Fat Using Silicic Acid Chromatography^{1, 2,}

Department of Dairy Science, Pennsylvania State University, University Park

ABSTRACT

Milk fat contains an almost infinite number of different triglycerides only a few of which have been separated in sufficient concentration to make positive identification possible. Attempts to establish the presence of specific triglycerides involve extensively fractionation of the fat, using a variety of methods. During the course of an investigation of a high-melting glyceride fraction (HMGF), a silicic acid chromatography method was developed that was particularly effective in concentrating the stearate-rich triglycerides. The method, herein described, was used to establish the presence of disteraryl triglycerides in milk fat.

HMGF was obtained by holding a solution of 400 g. of butteroil and two liters of acetone at -2· C. for 12 hr. The precipitate was washed with cold solvent, redissolved in 800 ml. of acetone, and held overnight at room temperature. The crystalline material recovered by filtration was washed with acetone. Evaporation of the occluded solvent yielded 20 g. of HMGF.

¹ Authorized for publication as Paper No. 2501 on October 20, 1960, in the Journal Series of The Pennsylvania Agricultural Experiment Station.

² Supported in part by U. S. Public Health Service (H3632).