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Journal of Dairy Science Vol. 44 No. 10 1811-1817
© 1961 by American Dairy Science Association ®
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Identification of Substances in Milk Cultures of Pseudomonas fluorescens which Stimulate Lactic Starter Cultures1

J. A. Koburger2 and T. J. Claydon

Department of Dairy Husbandry, Kansas Agricultural Experiment Station, Manhattan

ABSTRACT

Milk cultures of a strain of P. fluorescens incubated ten days at 30· C. were fractionated to identify the factor(s) which stimulated acid production by lactic starter cultures. Eight series of fractionations were made and the fractions tested on four lactic cultures. The fraction precipitated by acetone and ethanol increased titratable acidity an average of 28% over the control at 14 hr. of incubation at 21· C. This fraction also produced early coagulation of lactic starter cultures. Autoclaving the precipitate destroyed both the stimulatory activity and the early coagulation effect. Dialysis only slightly reduced the activity of the precipitate fraction. It appeared that enzyme activity was involved in stimulation, but this could not be clearly established. The amino acid fraction, which was stimulatory, contained 17 amino acids and gave an average increase in titratable acidity of 13%. Preliminary attempts to further purify the stimulatory factor from the precipitate fraction were unsuccessful.

FOOTNOTES

1 Contribution No. 296, Kansas Agricultural Experiment Station, Manhattan. Part of a dissertation submitted by the senior author as partial fulfillment of the requirements for the M.S. degree at Kansas State University.

2 Present address: Department of Animal Industry, North Carolina State College, Raleigh.






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Copyright © 1961 by the American Dairy Science Association ®.