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Gas Chromatographic Analysis of Milk Fat^{1, 2,}

Department of Dairy Science, The Pennsylvania Agricultural Experiment Station, University Park

ABSTRACT

Gas chromatography is one of those remarkable developments in science with far-reaching effects. Primarily, it is a means of separating mixtures of volatile organic compounds in a highly efficient manner.³ This principle is of such general importance that it is difficult to predict the many ultimate uses that gas chromatography may have. Although broad exploration of the method began about 5 yr. ago, it is already evident that gas chromatography will be indispensable in lipid analysis. At the moment, it is most appropriately applied in determining fatty acid composition of lipids through analysis of the methyl esters. Its use in the analysis of steroids and intact triglycerides is being probed in a number of laboratories. Although vapor pressures of such classes of compounds are discouragingly low at reasonable temperatures, some members of these groups have been gas chromatographed satisfactorily.

To date, milk lipids have not received extensive appraisal with this new analytical tool.

¹ Authorized for publication as Paper No. 2466 on June 17, 1960, in the Journal Series of the Pennsylvania Agricultural Experiment Station.

² Supported in part by the U. S. Public Health Service (H-3632).