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Survival Rates of Rapidly Frozen Bovine Spermatozoa

Department of Animal Husbandry, Cornell University, Ithaca, New York

ABSTRACT

Subsamples of semen from each of two ejaculates from each of 12 bulls were extended with a yolk-citrate-glycerol extender, equilibrated 5 to 6 hr. at 5° C., and placed directly in a freezing bath at temperatures of +5, 0, –5, –10, –15, –20, –25, –30, –35, and –40° C. The freezing bath was cooled at the rate of 0.8° C. per minute from +5 to –15° C., at a rate of 5° C. per minute from –15 to –40° C., and at the rate of 10° C. per minute from –40 to –75° C. The percentage of motile spermatozoa in ampules subsequently stored in a mechanical freezer at –85° C. for 60 days were, in order of decreasing temperature of the freezing bath at the time of transfer, 29, 21, 24, 27, 33, 36, 35, 35, 35, and 35. From these data it is concluded that time can be saved and a higher percentage of motile spermatozoa recovered by transferring the semen abruptly from +5° C. to a freezing bath ranging from –20 to –40° C.