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Fertility Results from the Use of Bovine Semen in a Carbon Dioxide Extender¹

Department of Animal Industry and Veterinary Science, University of Arkansas, Fayetteville

ABSTRACT

Increasing the fertile life of spermatozoa in liquid extender has been a significant problem in the field of artificial insemination. Numerous extenders have been used in an attempt to solve this problem. Petrov and Schneerson (3) stated that carbon dioxide in an undiluted ejaculate brought the semen into a relative state of anabiosis. The use of NAHCO₃ at O° C. was found by Schroder and Ramenskaja (5) to produce anabiosis. Milovanov and Hhabibulin (2) concluded anabiosis was brought about by the accumulation of CO₂ and its narcotizing effect on the spermatozoa. Trials conducted by Shettles (7) indicated that human spermatozoa could be completely immobilized by the use of CO₂. Motility was restored when the CO₂ was replaced by O₂ or air. Salisbury and VanDemark (4) concluded that the rate of glycolytic activity following inhibition with CO₂ was directly related to the completeness of the initial inhibition. A preliminary investigation on the use of the Illlinois Variable Temperature CO₂ extender was reported in 1957 by VanDemark and Sharma (9).

¹ Published with the approval of the director of the Arkansas Agricultural Experiment Station.