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Calcium and Magnesium Determination in Bovine Blood by Edta Titration

Department of Dairy Industry, Colorado State University, Fort Collins

ABSTRACT

This study was undertaken in an attempt to establish a method for calcium and magnesium determinations which would be rapid and accurate for the routine determinations of these ions in cows' blood. A procedure which does not require the removal of serum proteins would simplify these determinations. Several methods have been reported for the determination of blood serum calcium by titration with ethylenediaminetetraacetate (EDTA) (1, 4). A method for estimating blood levels of both calcium and magnesium with this reagent was reported by Todd (5), after completion of the trials reported here. The determination of calcium and magnesium in other biological materials, together with the complexometric reactions involved, has been reported (2, 6, 7).

A total of 43 blood samples were collected for calcium analyses from 26 different dairy cows and heifers of the Holstein, Jersey, and Guernsey breeds. No samples were included from cows affected with milk fever. Magnesium determinations were made on 23 samples obtained from 16 different cows and heifers.