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Metabolism of Volatile Fatty Acids by the Perfused Liver of Cows with Ketosis¹

Dairy Department, University of Maryland, College Park

ABSTRACT

A preliminary study of the biochemical aspects of bovine ketosis was conducted by perfusing with heparinized blood the livers from two mature cows affllicted with the disorder. Two lactating cows, each weighing approximately 1,200 lb., were selected for the study. These animals had a history of susceptibility to ketosis, and at the time of sacrifice exhibited the clinical and biochemical symptoms and signs of the disease. It was hoped that a comparison of results obtained in this investigation with those of previous perfusion studies of normal livers would give new insights into the metabolic aspects of bovine ketosis.

Experiments with both ketotic livers were essentially similar, except in regard to the C¹⁴-labeled acids which were added to the perfusate. In Perfusion A, 1.0 mc. of acetate-1-C¹⁴ Na was added to the blood, whereas 0.5 mc. of n-butyrate-1-C¹⁴ Na was added in Perfusion B. The perfusion procedure and methods of blood and tissue analysis were as previously reported in studies with goat livers (4).

¹ Scientific article No. A-808, contribution No. 3080 of the Maryland Agricultural Experiment Station.

² Present address: Department of Dairy Science, The Pennsylvania State University, University Park.

³ Present address: Food and Agricultural Organization of the United Nations, Rome, Italy.