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Some Factors Affecting the Growth of Streptococcus Bovis on Chemically Defined Media

Department of Dairy Science, University of Illinois, Urbana

ABSTRACT

Some factors which affect the growth of Streptococcus bovis in chemically defined media were studied. It was demonstrated that a substance produced by autoclaving glucose with phosphate is required for growth on NH₄Cl, but not on L-grutamine as a sole nitrogen source. This product of autoclaving was replaced by potential electron acceptors, i.e., acetaldehyde, acetoin, and pyruvate, or by lowering the initial pH of the medium below 7.0. The effects of anaerobiosis and the medium used to grow inocula on the growth of S. bovis in chemically defined media have been discussed. The amounts of NH₄Cl and L-glutamine required for growth have been compared in the presence and absence of L-glutamic acid. No synergistic growth response to combinations of a good nitrogen source, i.e., L-glutamine or NH₄Cl and L-glutamic acid, a poor nitrogen source, was observed.

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