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Metabolism of Bovine Semen. X. Oxidation of Carboxy-C¹⁴-Labeled Fatty Acids by Bovine Spermatozoa¹

Dairy Breeding Research Center, Department of Dairy Science, The Pennsylvania State University, University Park

ABSTRACT

Carbon¹⁴-labeled fatty acids have been used to show that washed bovine spermatozoa oxidize acetate, octanoate, and butyrate readily; the other acids tested (myristate, stearate, palmitate, and formate) were converted to C¹⁴O₂ at slower but appreciable rates. The production of C¹⁴O₂ from octanoate-1-C¹⁴ was shown to be oxygen-dependent and blocked by azide, cyanide, and malonate, but stimulated by 2, 4-dinitrophenol and coenzyme A. Glucose and glycerol did not appear to be competitive with octanoate as substrates for spermatozoa, but the presence of unlabeled acetate, propionate, or butyrate caused some reduction in utilization of octanoate-1-C¹⁴.

¹ Authorized for publication on January 25, 1960, as Paper No. 2429 in the journal series of the Pennsylvania Agricultural Experiment Station. Supported in part by grants from the Pennsylvania Association of Artificial Breeding Cooperatives, the National Association of Artificial Breeders, and the Atomic Energy Commission, Contract No. AT (30-1)-1849.