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Metabolism of Bovine Semen. IX. Glutamic-Oxaloacetic and Glutamic-Pyruvic Transaminase Activities¹

Dairy Breeding Research Center, Department of Dairy Science, The Pennsylvania State University, University Park

ABSTRACT

Assays on 63 samples of bovine semen gave an average of 623 ± 48 units of glutamic-oxaloacetic transaminase (GO-T) and 15 ± 1 units of glutamic-pyruvic transaminase (GP-T) per milliliter of seminal plasma. Statistically significant correlations of .849 for GO-T and .409 for GP-T were observed between seminal plasma transaminase activity and numbers of spermatozoa per milliliter of semen. When spermatozoa were freed of seminal plasma and the GO-T and GP-T activities in seminal plasma and cells compared, more activity was associated with the cells than with seminal plasma. With seminal plasma-free cells, GO-T per milligram of dry weight was similar to that reported in several bovine tissues, but lower than the activity in heart, liver, and skeletal muscle.

¹ Authorized for publication on January 27, 1960, as Paper No. 2430 in the journal series of the Pennsylvania Agricultural Experiment Station. Supported in part by grants from the Pennsylvania State Association of Artificial Breeding Cooperatives, the National Association of Artificial Breeders, and the Atomic Energy Commission, Contract No. AT(30-1)-1849.