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Chemical Stability of Disodium Phenyl Phosphate in Carbonate-Bicarbonate Buffer¹

Dairy Department, Clemson College, Clemson, South Carolina

ABSTRACT

Samples of disodium phenyl phosphate substrate were heat treated or autoclaved and stored with unheated and chloroform-preserved samples at 5 and 20° C. for 1 yr. Aliquots were removed from storage at regular intervals for estimation of free phenol, using N, 2, 6-trichloro-p-quinoneimine (CQC) as indicator. Free phenol was found in all unheated samples after 2 wk. of storage. No free phenol was found in any of the heat-treated or autoclaved samples after 1 yr. of storage. The heat treatment and age of the buffer-substrate had no effect on the sensitivity of the phosphatase method.

¹ Technical contribution No. 321, South Carolina Agricultural Experiment Station, Clemson Agricultural College, Clemson, South Carolina.