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Fatty Acid Requirements of Certain Rumen Bacteria¹

Department of Bacteriology, University of Wisconsin, Madison

ABSTRACT

Cultures were isolated from 10⁸ dilutions of rumen material in roll tubes of a trypticase-phytone-glucose basal medium supplemented with rumen fluid. Almost one-fourth of the isolates failed to grow on subculture in the basal medium, unless it was enriched with rumen fluid or a mixture of fatty acids commonly found in the rumen.

In further trials with five representative isolates, all cultures grew well with a combination of n-valerate and isovalerate replacing the rumen fluid or the fatty acid mixture. None grew with n-valerate as the sole additive to the basal medium, but all grew appreciably with isovalerate. Pour of the five grew considerably better with a mixture of the two acids than with isovalerate alone. One of the cultures was tentatively identified as a Borrelia species and two as Bacteroides succinogenes. The two other organisms were not identified as recognized species.

¹ Published with permission of the Director, Wisconsin Agricultural Experiment Station.