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Journal of Dairy Science Vol. 43 No. 2 250-255
© 1960 by American Dairy Science Association ®
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Influence of Yolk-Citrate, Yolk-Glycine, Whole Milk, and Skimmilk Extenders Containing Glycerol on Motility of Bovine Sperm at 37 and 5° C.1,2,

J. L. Albright3, R. E. Erb and M. H. Ehlers

Department of Dairy Science, Washington State University, Pullman

ABSTRACT

Sixteen semen samples were extended 1: 50 in 14 extenders with 7.5% glycerol added by direct and step-wise procedures to give 28 different extender treatments. Aliquots of each treatment were stored at 37° C. for 12 hr. and at 5° C. for seven days. Yolk-2.9% citrate, yolk-2.0% citrate, yolk-2.9% glycine, whole milk, and skimmilk were the basic extenders, together with mixtures of these at 1:1 in all possible pair combinations. Direct glycerolation generally gave better results than stepwise, as evaluated by progressive sperm motility, but these effects were not significant by seven days of storage at 5° C. Independent of glycerolation method, progressive motility at 37° C. was significantly higher in yolk-2.9% citrate and its combinations than in yolk-2.0% citrate and its combinations, but the reverse was true at 5° C. Extenders composed solely of milk constituents performed favorably at 5° C. and poorly with prolonged 37° C. storage. No single extender or combination of two basic extenders gave similar results, as time and temperature of storage and method of adding glycerol were varied.

FOOTNOTES

1 Scientific Paper No. 1792, Washington Agricultural Experiment Station, Pullman. Project 1107.

2 The data are from a thesis presented by the senior author to the graduate faculty of the State College of Washington in partial fulfillment of the requirements for the Ph.D. degree.

3 Present address: Department of Dairy Science, University of Illinois, Urbana.






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