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Journal of Dairy Science Vol. 43 No. 2 175-186
© 1960 by American Dairy Science Association ®
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A Quantitative Test for Carrageenin Ester Sulphate in Milk Products1

P. M. T. Hansen and R. McL. Whitney

Department of Food Technology, University of Illinois, Urbana

ABSTRACT

A method was developed to determine gravimetrically the concentration of carrageenin in some milk products by the sulphate content of a dialyzed milk-carrageenin system after hydrolysis with hydrochloric acid and decolorization with activated carbon. The effects of the variables involved were studied. The method was employed to determine the ester-sulphate content of the carrageenin anions of various salts of {kappa}- and {lambda}-carrageenin. The results obtained for the {lambda}-carrageenin were in agreemnt with those predicted from the commonly accepted structure of this fraction, whereas the value for the {kappa}-fraction suggested a slight alteration in its reported structure.

It is suggested that this method could also be used to determine the sulphate content of milk if the dialysis step were omitted.


FOOTNOTES

1 Taken from the thesis of P. M. T. Hansen presented in October, 1958, to the University of Illinois, Urbana, in partial fulfillment of the requirements for the Master of Science degree.







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Copyright © 1960 by the American Dairy Science Association ®.