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Technique for Obtaining Serial Samples of Arterial Blood from the Middle Coccygeal Artery in the Bovine¹

Department of Dairy Husbandry

W. W. Crenshaw

Department of Veterinary Anatomy University of Missouri, Columbia

ABSTRACT

Studies dealing with the physiological variations in the relative amounts of various blood constituents in arterial and venous blood have brought about the need for a convenient technique for obtaining serial samples of arterial blood.

Blackwood and Stirling (1) took arterial blood from the radial artery of lactating and nonlactating cows, but found arterial puncture of nonanesthetized cows to be rather difficult. Lintzel (5) reported that he obtained arterial blood from goats and cattle by puncture of the heart chamber. Graham et al. (3) obtained arterial blood from the internal iliac artery, but had to puncture the rectal wall, with the resulting possibility of infection into the blood stream. Graham et al. (4) described an operation by which the carotid artery was exteriorized and sewed into a skin flap which gave a convenient place to obtain arterial blood. Boyd and Petersen (2) reported a technique for puncture of the external pudic artery.

¹ Contribution from the Missouri Agricultural Experiment Station, Journal Series No. 2190. Approved by the Director.