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Department of Dairy Science, University of Illinois, Urbana
ABSTRACT
On the basis of several categories of evidence which indicate that the C02 reduction system is the major pathway of rumen methanogenesis, it can be postulated that the ratio of C02: CH4 in rumen gas can be altered by increasing the H2 pressure in the rumen. Similarly, if acetate appreciably contributes to rumen methane, the addition of D-limonene, which inhibits acetate-utilizing methane bacteria (1, 2), also should alter the C02:CH4 ratio. Data from experiments designed to measure the ratios of C02: CH4 in rumen gas as affected by ration and digestive chronology, and to describe the effects of added H2 and D-limonene on these ratios, are reported herein.
All trials were performed with a 12-yr.-old Guernsey cow except those involving the use of H2, which were conducted on a 4-yr.-old Holstein cow. Both animals were fitted with a plastic cannula. Feed was withheld for 12 hr. prior to each trial.
1 N.I.R.D., Shinfield, Reading, England.
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