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Journal of Dairy Science Vol. 43 No. 10 1471-1475
© 1960 by American Dairy Science Association ®
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Improved Photometric Method for Assaying Phenolsulfonphthalein in the Blood of Cattle in Tests for Renal Function1

R. R. Anderson2 and J. P. Mixner

Dairy Science Department, New Jersey Agricultural Experiment Station, Sussex

ABSTRACT

The usual photometric procedures for the assay of phenolsulfonphthalein (PSP) in plasma containing free hemoglobin in renal function studies have been found to be inadequate. An improved method of photometric assay for PSP in plasma has been developed in which plasma blank optical densities (O.D.) of a series of blood samples containing no dye, but with varying degrees of induced hemolysis, were measured both in acidic and in basic media. Basic plasma blank O.D. were predicted from acidic plasma blank O.D. by means of derived regression equations. In the routine assay of PSP in plasma, O.D. readings were first made on the acidified plasmas. Excess base was then added and O.D. readings again made on the sample. When the predicted basic plasma blank O.D. were used to correct the O.D. of the dye-containing plasma samples (varying amounts of hematin), accurate estimates of dye content were obtained. In actual renal function tests in both calves and cows the improved photometric method was superior to the original procedure in the precision with which the measures were made.

FOOTNOTES

1 Paper of the Journal Series, New Jersey Agricultural Experiment Station, Rutgers—The State University, New Brunswick. This work was supported in part by a grant from Merck Sharp & Dohme Research Laboratories, Division of Merck & Company, Rahway, New Jersey.

2 Present address: Department of Dairy Husbandry, University of Missouri, Columbia.






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