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A Comparison of the All-Glass, Semipermeable Membrane, and Continuous Flow Types of Apparatus for In Vitro Rumen Fermentations^{1, 2,}

Department of Animal Science, Ohio Agricultural Experiment Station, Wooster

ABSTRACT

A comparison was made of three different types of apparatus commonly used for in vitro rumen fermentation studies. The types compared were the all-glass, the semipermeable membrane, and the continuous flow. Purified wood cellulose and several different types of hay were used as substrates, with cellulose digestion, total volatile fatty acid production, and ammonia nitrogen production (for the hay samples only) being used as criteria of microbial activity. No major differences were found between the different types of apparatus; however, the all-glass apparatus appears to be advantageous because of its simplicity.

¹ Approved for publication as Journal Article No. 75–59 by the Associate Director of the Ohio Agricultural Experiment Station, Wooster.

² This work was supported in part by funds allocated to the Station on the recommendation of the North Central Technical Committee on Ruminant Nutrition (NC-25).

³ On leave from the Faculty of Agriculture, University of Alexandria, Alexandria, Egypt.