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Physical and Chemical Changes in Bovine Saliva and Rumen Liquid with Different Hay-grain Rations^{1, 2,}

Departments of Dairy, Microbiology, and Agricultural Chemistry, Michigan State University, East Lansing

ABSTRACT

Saliva and rumen liquid samples were collected from two cows that were fed 20, 40, 60, 80, and 100% hay rations. The viscosity of saliva (mean 99 c.p.) was greater 3 hr. after feeding than before feeding and the pH (mean 8.2) increased as the proportion of hay increased. The ash of saliva (mean 0.73%) was greater with the high-grain rations. Total salivary nitrogen was 20.1 mg. % for the 20% hay ration and averaged 14.9 mg. % for the 40 to 100% hay rations. Hexosamines (mean 4.3 mg. %) were higher on the high-grain rations. The rumen liquid samples were centrifuged at 25,000 x G. and the supernatant analyzed. The mean dry matter content was 1.71% and the ash content was 0.79%. Sodium (mean 234 mg. %) increased as the hay content increased and was higher for the cow which tended to bloat than for the one which did not. Hexosamines (mean 3.7 mg. %) were also greater for the bloaty cow, but tended to increase in both cows with increasing proportions of grain. The total nitrogen of rumen liquid (mean 37.1 mg %) was highest on the 20% hay rations, was higher after feeding than before, and was higher for the bloaty cow than for the one which did not bloat.

¹ Published with the approval of the Director of the Michigan Agricultural Experiment Station as Journal Article No. 2393.

² Supported in part by funds provided through the North Central Regional Project NC-27.

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