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Metabolism of Bovine Semen. VIII. Fluoroacetate as an Inhibitor of Spermatozoan Motility, Glycolysis, and Respiration^{1, 2,}

Dairy Breeding Research Center, Department of Dairy Science, The Pennsylvania State University, University Park

ABSTRACT

Sodium fluoroacetate in concentrations as high as 0.01 M has been tested as an inhibitor of the motility, glycolysis, and respiration of bovine spermatozoa in heated skimmilk, egg yolk-citrate (1:3), and modified Ringer solution. Effective inhibition of motility and glycolysis was not obtained. Endogenous respiration was 70% inhibited by 0.01 M fluoroacetate; respiration with substrates of 0.01 M acetate, glucose, and fructose was inhibited 70–90%. Production of C¹⁴O₂ from acetate-1-C¹⁴ was almost completely blocked by fluoroacetate.

¹ Authorized on May 4, 1959, for publication as paper No. 2359 in the journal series of the Pennsylvania Agricultural Experiment Station.

² Supported in part by grants from the Pennsylvania State Association of Artificial Breeding Cooperatives, the National Association of Artificial Breeders, and the Atomic Energy Commission, Contract No. AT(30-1)-1849.

³ Part of the data contained in this paper are from a thesis submitted by the senior author to the Graduate School of The Pennsylvania State University in partial fulfillment of the requirements for the degree of Master of Science.