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Effects of Glycerol and Fructose on Livability, Motility, and Anaerobic Gas Production of Bovine Spermatozoa¹

Department of Dairy Husbandry, University of Missouri, Columbia

ABSTRACT

In vitro manometric determinations of gas production by bovine spermatozoa under anaerobic conditions indicated a slight but statistically nonsignificant inhibitory effect of glycerol. The inclusion of 1% fructose with 1.0, 3.3, or 10.0% glycerol caused an immediate and marked stimulation of gas production by the spermatozoa.

Glycerol plus fructose maintained a greater degree of motility and livability of bovine spermatozoa at the end of 105 min. of incubation at 38° C. than did either fructose or glycerol alone. Under the conditions of this study, 1% glycerol plus 1% fructose was the apparent optimum for maintenance of spermatozoan motility and livability.

¹ Contribution from the Missouri Agricultural Experiment Station, Journal Series No. 1956. Approved by the Director.

² Present address: Department of Animal Industries, University of Connecticut, Storrs.