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Journal of Dairy Science Vol. 42 No. 7 1216-1222
© 1959 by American Dairy Science Association ®
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Influence of Prolonged Incubation of Bull Semen at 37° C. On Fructolysis, Lactic Acid, and Motility1

R. E. Erb, J. L. Albright2 and M. H. Ehlers

Department of Dairy Science, State College of Washington, Pullman

ABSTRACT

Eighteen semen samples, three from each of six dairy bulls, were concentrated by centrifuging and removing supernatant plasma to one-half of the original volume. The sperm and remaining plasma were extended 1:6 with 2.9% Na-citrate and divided into 20 tubes of 1 ml. and incubated at 37° C. Duplicate tubes were removed at 0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 4.0, 5.0, and 6.0 hr. of incubation, for determination of pH, fructose, lactic acid, and motility rating on a 0 to 10 scale (10 = 100%). At 0 hr. of incubation the averages were: (a) pH—6.87; (b) fructose—4.27 ± 0.33 mg/ml of semen; (c) lactic acid—0.84 ± 0.10 mg/ml of semen, and (d) motility—6.56 ± 0.89. pH remained essentially unchanged during incubation. Fructose utilization by 109 sperm/ml/hr was 2.02 ± 0.18 mg. during the first 0.5 hr. of incubation. Motility ceased when fructose concentration was less than 0.06 mg/ml of extended semen and was seriously impaired at observed levels of 0.14 to 0.29 mg/ml. Lactic acid accumulation reached levels of 0.6 mg/ml of extended semen, but was not a principal cause of reduced motility. It is recommended that fructose levels in metabolism studies be expressed as mg/ml of extended semen, as well as the concentration per milliliter of whole semen, since different extension rates are frequently used.

FOOTNOTES

1 Scientific Paper No. 1783, Washington Agricultural Experiment Station, Pullman, Project 1107.

2 Present address: California Polytechnic College, San Luis Obispo.






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Copyright © 1959 by the American Dairy Science Association ®.