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Influence of Temperature History on the Response of Psychrophiles to Different Incubation Temperatures

Dept. of Bacteriology, Southwestern Louisiana Institute, Lafayette

ABSTRACT

The routine bacteriological technique of incubating cultures at temperatures suitable for growth followed by refrigerating to terminate growth presents a frustrating problem to the investigator of psychrophilic bacteria. With these organisms, one is faced with a sequence of two actual incubation periods—the first suitable for rapid proliferation, and the second suitable for high viable counts (1). This sequence of incubations might influence the subsequent behavior of the culture. Sherman and Cameron (3) have shown that coliform bacteria lost their viability when transferred from 45 to 10° C; whereas, cultures originally grown at 10° C. retained their viability when subcultured at 45° C. Olson and Schultze (2) studied psychrophiles and reported that the relative ability of their cultures to oxidize glucose at three different temperatures was a function of the temperature at which the cultures previously had been grown.

To study the possibility that propagation temperature history might influence the behavior of subcultures, a series of simple trials was performed using a typical psychrophile, a proteolytic strain of Pseudomonas fluorescens isolated from cottage cheese.