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Effect of Feeding N,N'-Diphexyl-Para-Phenylenediamine (DPPD) to Lactating Dairy Cows on the Production of Butterfat¹

Storrs (Conn.) Agricultural Experiment Station

ABSTRACT

Slight increases in the butterfat percentage were observed in three out of four experiments (3, 10, 11) when N,N'-diphenyl-para-phenylenediamine (DPPD) was included in the ration of lactating cows for the purpose of inhibiting copper-induced oxidized milk flavor. These results suggested that DPPD, when fed to lactating cows, may affect lipid metabolism. However, no definite conclusions could be drawn, since feed intakes were not rigidly controlled or a system of equalized feeding was not employed in the above-mentioned experiments. The present study was, therefore, initiated to determine the effect of feeding DPPD on the butterfat percentage of the milk of lactating dairy cows which were fed equivalent energy for maintenance and production. In addition, DPPD was determined in the milk.

Twelve cows, two Ayrshires, six Holsteins, and four Jerseys, were paired according to breed and insofar as possible according to age and days in lactation. Each cow of a pair was assigned randomly to one of two sequences of including DPPD in the ration, namely: DPPD—No DPPD—DPPD and No DPPD—DPPD—No DPPD, designated as Sequence A and Sequence B, respectively.

¹ This study was made in part with funds provided by the Chas. H. Hood Dairy Foundation, Boston, Mass., and Wirthmore Feeds, Inc., Waltham, Mass. The authors are grateful to G. Farrington and Mrs. Mae Miller for technical assistance, to Dr. H. J. Fisher, Connecticut Agricultural Experiment Station, New Haven, for proximate analyses of the feeds, and to M. E. Morgan and H. D. Eaton for helpful suggestions.

² Present address: Experimental Statistics and Animal Industry Departments, North Carolina State College, Raleigh.

³ Present Address: Animal Science Department, Ohio Agricultural Experiment Station, Wooster.