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Determination of Bovine Serum Calcium with a Simple Flame Photometer¹

Storrs (Connecticut) Agricultural Experiment Station

ABSTRACT

A large number of methods are available for the determination of serum calcium by flame photometry; however, these are fairly specific for the type of instrument used (1). Baird Associates, Inc. have distributed a tentative procedure for the determination of serum calcium, utilizing the Baird flame photometer.² While this procedure appeared advantageous in regard to time and ease of manipulation, no comparisons with accepted calcium methods were available. In principle, this tentative procedure is similar to several existing methods (1), in that anion interference is avoided through prior precipitation of calcium as the oxalate.

¹ The author is indebted to H. D. Eaton for helpful suggestions in the preparation of the manuscript, and to G. S. Meyers, Jr. and D. G. Hazzard for technical assistance.

³ Present address: Department of Animal Science, Ohio Agricultural Experiment Station, Wooster.