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Metabolism of Bovine Semex. VII. Pyruvate-Alanine Conversion¹

Dairy Breeding Research Center, Department of Dairy Science, The Pennsylvania State University, University Park

ABSTRACT

Conversion of pyruvate to alanine and the reverse reaction have been demonstrated in dialyzed extracts of bovine spermatozoa and of seminal plasma, and in intact washed spermatozoa. Using washed spermatozoa, substituting Ringer phosphate-glycine for Ringer phosphate, results in an increase in alanine and a decrease in pyruvate. It is suggested that the glycine-containing buffer forces the transamination reaction

to the right, and that this reaction accounts for the reduced accumulation of lactate in diluents containing glycine.

¹ Authorized for publication on December 11, 1958, as Paper No. 2326 in the journal series of the Pennsylvania Agricultural Experiment Station. Supported in part by grants from the Pennsylvania State Association of Artificial Breeding Cooperatives, the National Association of Artificial Breeders, and the Atomic Energy Commission, Contract No. AT(30-1)-1849.