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Determination of Phosphorus in Milk Lipids

Department of Dairy Industry, University of California, Davis

ABSTRACT

Investigation of milk lipids of ten requires determination of the phosphorus in residues from extracts such as those obtained by the Mojonnier procedure or from chromatographic eluates. Colorimetric procedures generally are preferred to titrimetric or gravimetric methods, as being faster and better adapted to analyzing small amounts of material. In our laboratory we have used a modification of the method of Harris and Popat (2) for determining phosphorus in cottonseed lipids. The method employs perchloric acid for sample digestion and p-methylaminophenol sulfate (elon) as a reducing agent for color development. Both of these steps are performed in the same test tube, to avoid transfer losses.

In developing their method, Harris and Popat investigated the effect of a number of variables on the digestion of lipid samples and on color development with elon. They stated that the oxidation and digestion procedure can be completed within 5 min. However, they did not report any data showing the effect of varying the time of digestion, although they emphasized that the final clariflcation step in the digestion procedure must be complete.