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Journal of Dairy Science Vol. 42 No. 3 550-552
© 1959 by American Dairy Science Association ®
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Electrophoresis of Unfractionated Proteins from Homogenized Whole Milk

J. Tobias1 and R. M. Serf2

ABSTRACT

A procedure is described consisting of dialysis of homogenized whole milk against 0.1 M NaCl and ultracentrifugation at 40,000 x G, by which it is possible to obtain a suspension of unfractionated milk proteins suitable for electrophoretio analysis. No changes in mobilities or relative concentrations of electrophoretic components at pH 8.7 and 0.1 µ could be attributed to this manner of sample preparation.

A question is raised as to the identity of a small peak observed in the electrophoretic patterns of homogenized whole milk which moved with a slightly higher mobility than {alpha}-casein.

FOOTNOTES

1 Department of Food Technology, University of Illinois, Urbana.

2 Present address: Foremost Dairies, Inc., San Francisco, California.






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Copyright © 1959 by the American Dairy Science Association ®.