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The Carbohydrates of Some Glycoproteins of Bovine Milk¹

Department of Dairy, Food Technology, Michigan Agricultural Experiment Station, East Lansing

ABSTRACT

The presence of carbohydrates in proteins has been recognized for many years. Recent studies have demonstrated that many of the blood and human milk proteins contain appreciable quantities of hexoses, hexosamines, fucose, and/or an interesting amino sugar—"sialic" or "neuraminic acid" (9). Evidence indicating the presence of glycoproteins in milk was furnished first by the work of Storch in 1897 (7), who reported that the fat globule membrane contained a carbohydrate. Recently, Nitschmann (5) characterized a macro-peptide which was released from casein following a rennin treatment. This component, soluble in 12% TCA, contained 15.2% galactose, 4.3% glucosamine, and 11.4% neuraminic acid (1).

The objective of our studies has been to identify the carbohydrates which are chemically bound to a) the soluble protein fraction of the fat globule membrane (4), b) the minor protein fraction as reported by Weinstein (8), and c) the proteose-peptone fraction (6). Chemical and chromatographic techniques were employed. Protein-bound hexose, hexosamine, fucose, and sialic acid were determined according to the analytical scheme outlined by Glick (3).

¹ Michigan Agricultural Experiment Station Journal Article No. 2340.