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Comparison of Glass Ampules and Plastic Vials Based on the Nonreturn Rate of Frozen Bovine Spermatozoa

Department of Dairy Husbandry, Institute of Institute of Agriculture^,2, University of Minnesota, St. Paul

ABSTRACT

Many different types of containers have been used for the storage of frozen bovine spermatozoa. Few data have been reported on the effect plastic vials or glass ampules may have on the fertility of frozen semen. VanDemark and Kinney (9) found no significant difference in the freezability of five semen samples frozen in glass ampules or plastic vials. Brugman and Schmidt (1) reported on a limited number of animals bred and found that fertility was not altered by type of container. Dunn et al. (2) reported on experiments conducted on a splitsample technique to compare the fertility of frozen semen stored at –79° C. in hermetically sealed glass ampules and in polyethylene bulbs. A total of 298 inseminations were made with semen frozen in glass ampules, and 313 inseminations were made with semen frozen in the polyethylene bulbs. The 60- to 90-day nonreturn rates were 60.7 and 62.3%, respectively.

¹ Present address: Tri-State Breeders' Cooperative, Wetsby, Wisconsin.

² Paper No. 3918 Scientific Journal Series, Minnesota Agricultural Experiment Station.