HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Simmons, J. C. Articles by Graham, D. M. Search for Related Content PubMed Articles by Simmons, J. C. Articles by Graham, D. M.

Maintenance of Active Lactic Cultures by Freezing as an Alternative to Daily Transfer

South Carolina Agricultural Experiment Station, Clemson

ABSTRACT

Daily propagation of lactic cultures involves considerable expenditure of time, media, and materials, and requires careful technique to avoid contamination during repeated transfers. Furthermore, the desirable balance of strains generally present in a freshly combined culture frequently is lost after several propagations. The preservation of an active culture in its optimum condition for flavor and acid production has been attempted by Johns (3), Olson (5), and Heineman (1). This report deals with the technique studied by the writers in a similar attempt to maintain lactic cultures at optimum activity without daily transfers.

Cultures were prepared by inoculating a sterile milk medium at the rate of 1% with freshly ripened active starter. The inoculated milk was placed at once in an ice cream-hardening room at –20° F. After storage for up to 6 mo., the previously inoculated milk was thawed in about 2 hr. in a water bath at 70° F., then incubated for 16 hr. at 70° F.

² Dairy Industry Supply Association Fellow.

³ Present address: Pet Milk Company, Research Laboratory, Greenville, Illinois.